Human Melanophilin - MLPH ELISA Kit

Mfr.No.

E4007hu

Description

This sandwich kit is for the accurate quantitative detection of human Melanophilin (also known as MLPH) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Exophilin-3;Melanophilin;MLPH;SlaC2-a;Slp homolog lacking C2 domains a;Synaptotagmin-like protein 2a

Reactivity

Human

Detection Range

10-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

65949

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.17ng/L

Background

Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.

Gene ID	79083
UniProt ID	Q9BV36
Antigen	MLPH

Research Area

Others

Protocol

These standard curves of E4007hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.11	2.104	2.061
	2.098
800ng/L	1.046	0.995	0.952
	0.944
400ng/L	0.48	0.495	0.452
	0.51
200ng/L	0.256	0.219	0.175
	0.181
100ng/L	0.091	0.114	0.071
	0.138
0ng/L	0.028	0.043	0
	0.058

Precision

We measured random samples of E4007hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	939.1	49.43	5.3
Intra-Assay	2	18	1286.4	30.01	2.3
Intra-Assay	3	18	290.2	15.3	5.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105434	E4007hu	48T	$320		Add to Cart
BHE12105434	E4007hu	96T	$458		Add to Cart

Human Melanophilin - MLPH ELISA Kit

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