Human Melanoma-associated Antigen 3

Mfr.No.

E5655Hu

Sensitivity

0.151ng/mL

Detection Range

0.3-90ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Antigen MZ2-D;Cancer/testis antigen 1.3;CT1.3;MAGE-3 antigen;MAGEA 3;MAGEA3;Melanoma-associated antigen 3

Molecular Weight

34747

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5655Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	14.1	0.58	4.1
Intra-Assay	2	18	23.4	0.92	4
Intra-Assay	3	18	32.3	1.01	3.1

Background

Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

Protocol

These standard curves of E5655Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	2.069	1.967	1.924
	1.866
24ng/mL	1.098	1.098	1.055
	1.097
12ng/mL	0.447	0.516	0.473
	0.584
6ng/mL	0.166	0.176	0.133
	0.185
3ng/mL	0.082	0.122	0.079
	0.161
0ng/mL	0.033	0.043	0
	0.053

Gene ID	4102
UniProt ID	P43357

Research Area	Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107017	E5655Hu	48T	$320		Add to Cart
BHE12107017	E5655Hu	96T	$458		Add to Cart

Human Melanoma-associated Antigen 3 - MAGE-3 ELISA Kit

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