Human Melanoma-associated antigen 1(MAGEA1) ELISA kit

Mfr.No.

CSB-EL013323HU

Sensitivity

0.039 ng/ml

Detection Range

0.156 ng/ml-10ng/ml

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Recovery

The recovery of human KLK 10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-100
EDTA plasma (n=4)	95	90-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human KLK 10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-106
1:2	Average %	91
	Range %	88-97
1:4	Average %	95
	Range %	90-103
1:8	Average %	95
	Range %	89-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Cell lysates, Tissue homogenates

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<10%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

UniProt ID	P43355
Tissue Specificity	Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. Never expressed in kidney tumors, leukemias and lymphomas.

Applications	ELISA
Research Area	MAGEA1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503315	CSB-EL013323HU	96T	$695	Add to Cart
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BHE10503315	CSB-EL013323HU	10x96T	$6602.50	Add to Cart