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Home / ELISA Kit

Human Melanocyte protein PMEL (SILV) ELISA Kit

SKU : BHE13702874

Melanocyte protein PMEL (SILV) encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. Premelanosome protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. Premelanosome protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of premelanosome protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.

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Mfr.No.	KTE60658
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Melanocyte protein PMEL (SILV) ELISA Kit employs a two-site sandwich ELISA to quantitate SILV.
Alternative Names	SILV; D12S53E; ME20; PMEL; PMEL17; SI; SIL; gp100; Melanocyte protein mel 17; Pmel 17; Silver; mouse; homolog of; melanosomal matrix protein17; silver (mouse homolog)-like; silver homolog
Detection Method	Colorimetric
Kit Components	Human Melanocyte protein PMEL microplate Human Melanocyte protein PMEL standard Human Melanocyte protein PMEL detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Melanocyte protein PMEL (SILV) ELISA Kit has high sensitivity and excellent specificity for detection of Human SILV. No significant cross-reactivity or interference between Human SILV and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Melanocyte protein PMEL (SILV) encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. Premelanosome protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. Premelanosome protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of premelanosome protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.
Notes	This Human Melanocyte protein PMEL (SILV) ELISA Kit employs a two-site sandwich ELISA to quantitate SILV in samples. An antibody specific for SILV has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySILV present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SILV is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SILV bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6490

Applications	ELISA
Applications Notes	This Human Melanocyte protein PMEL (SILV) ELISA Kit employs a two-site sandwich ELISA to quantitate SILV in samples. An antibody specific for SILV has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySILV present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SILV is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SILV bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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