Human melanocyte antibody,MC Ab ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08521h

Sensitivity

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Detection Range

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Reactivity

Human

Sample Type

Serum

Assay Duration

1-5h

Assay Type

Indirect

Alternative Names

melanocyte antibody,MC Ab

Recovery

The recovery of mouse GRP78 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-98
EDTA plasma (n=4)	96	94-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GRP78 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-98
1:2	Average %	98
	Range %	93-104
1:4	Average %	89
	Range %	85-93
1:8	Average %	90
	Range %	87-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Antigen	melanocyte antibody,MC Ab

Applications	ELISA
Research Area	MC Ab

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Evaluation of serum immunoglobulins concentrations and distributions in vitiligo patients. Li Y.et al,Immunol Res.,Jan 7

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