Human melanin ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14051h

Sensitivity

15.6 pg/mL

Detection Range

12.5 pg/mL-1000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

melanin

Recovery

The recovery of rabbit IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-96
EDTA plasma (n=4)	92	89-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	101
	Range %	94-105
1:2	Average %	97
	Range %	91-102
1:4	Average %	89
	Range %	85-94
1:8	Average %	90
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	melanin

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Comparison of the global gene expression profiles in the bovine endometrium between summer and autumn. Sakumoto R. et al,J Reprod Dev,2015 61(4):297-303.

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