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Home / ELISA Kit

Human Meiotic recombination protein SPO11 (SPO11) ELISA Kit

SKU : BHE13702550

SPO11 is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described. The human and the mouse SPO11 proteins share 82% amino acid identity, but only 20 to 30% with other eukaryotic homologs such as Drosophila and C. elegans. Both the mouse and human SPO11 exons 2 and 8 are subject to alternative splicing. Northern blot analysis showed that mouse Spo11 is expressed in testis and thymus as a 1.8-kb mRNA, whereas human SPO11 is expressed in testis as a 2.0-kb transcript. RT-PCR revealed that human SPO11 is also expressed in prostate, fetal testis, thymus, and some carcinoma cell lines.

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Mfr.No.	KTE60333
Description	This Human Meiotic recombination protein SPO11 (SPO11) ELISA Kit employs a two-site sandwich ELISA to quantitate SPO11.
Alternative Names	SPO11; CT35; MGC39953; SPO11 meiotic protein covalently bound to DSB-like; SPO11; meiotic protein covalently bound to DSB-like; cancer/testis antigen 35; meiotic recombination protein SPO11
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Meiotic recombination protein SPO11 microplate Human Meiotic recombination protein SPO11 standard Human Meiotic recombination protein SPO11 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Meiotic recombination protein SPO11 (SPO11) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPO11. No significant cross-reactivity or interference between Human SPO11 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SPO11 is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described. The human and the mouse SPO11 proteins share 82% amino acid identity, but only 20 to 30% with other eukaryotic homologs such as Drosophila and C. elegans. Both the mouse and human SPO11 exons 2 and 8 are subject to alternative splicing. Northern blot analysis showed that mouse Spo11 is expressed in testis and thymus as a 1.8-kb mRNA, whereas human SPO11 is expressed in testis as a 2.0-kb transcript. RT-PCR revealed that human SPO11 is also expressed in prostate, fetal testis, thymus, and some carcinoma cell lines.
Notes	This Human Meiotic recombination protein SPO11 (SPO11) ELISA Kit employs a two-site sandwich ELISA to quantitate SPO11 in samples. An antibody specific for SPO11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPO11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPO11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPO11 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	23626
Antigen	SPO11
Tag	SPO11
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Meiotic recombination protein SPO11 (SPO11) ELISA Kit employs a two-site sandwich ELISA to quantitate SPO11 in samples. An antibody specific for SPO11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPO11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPO11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPO11 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702550	KTE60333	48 T	$339		Add to Cart
BHE13702550	KTE60333	96 T	$589		Add to Cart
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BHE13702550	KTE60333	96 Tx50	$24569		Add to Cart

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