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Home / ELISA Kit

Human Megakaryocyte-associated tyrosine-protein kinase (MATK) ELISA Kit

SKU : BHE13703927

MATKis an enzyme has amino acid sequence similarity to Csk tyrosine kinase and has the structural features of the CSK subfamily: SRC homology SH2 and SH3 domains, a catalytic domain, a unique N terminus, lack of myristylation signals, lack of a negative regulatory phosphorylation site, and lack of an autophosphorylation site.This protein is thought to play a significant role in the signal transduction of hematopoietic cells. It is able to phosphorylate and inactivate Src family kinases, and may play an inhibitory role in the control of T-cell proliferation. This protein might be involved in signaling in some cases of breast cancer. Three alternatively spliced transcript variants that encode different isoforms have been described for this gene.

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Mfr.No.	KTE61712
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Megakaryocyte-associated tyrosine-protein kinase (MATK) ELISA Kit employs a two-site sandwich ELISA to quantitate MATK.
Alternative Names	MATK; CHK; CTK; DKFZp434N1212; HHYLTK; HYL; HYLTK; Lsk; MGC1708; MGC2101; Csk-homologous kinase; Csk-type protein tyrosine kinase; HYL tyrosine kinase; hematopoietic consensus tyrosine-lacking kinase; hydroxy
Detection Method	Colorimetric
Kit Components	Human Megakaryocyte-associated tyrosine-protein kinase microplate Human Megakaryocyte-associated tyrosine-protein kinase standard Human Megakaryocyte-associated tyrosine-protein kinase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Megakaryocyte-associated tyrosine-protein kinase (MATK) ELISA Kit has high sensitivity and excellent specificity for detection of Human MATK. No significant cross-reactivity or interference between Human MATK and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MATKis an enzyme has amino acid sequence similarity to Csk tyrosine kinase and has the structural features of the CSK subfamily: SRC homology SH2 and SH3 domains, a catalytic domain, a unique N terminus, lack of myristylation signals, lack of a negative regulatory phosphorylation site, and lack of an autophosphorylation site.This protein is thought to play a significant role in the signal transduction of hematopoietic cells. It is able to phosphorylate and inactivate Src family kinases, and may play an inhibitory role in the control of T-cell proliferation. This protein might be involved in signaling in some cases of breast cancer. Three alternatively spliced transcript variants that encode different isoforms have been described for this gene.
Notes	This Human Megakaryocyte-associated tyrosine-protein kinase (MATK) ELISA Kit employs a two-site sandwich ELISA to quantitate MATK in samples. An antibody specific for MATK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMATK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MATK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MATK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4145

Applications	ELISA
Applications Notes	This Human Megakaryocyte-associated tyrosine-protein kinase (MATK) ELISA Kit employs a two-site sandwich ELISA to quantitate MATK in samples. An antibody specific for MATK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMATK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MATK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MATK bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703927	KTE61712	48 T	$339		Add to Cart
BHE13703927	KTE61712	96 T	$589		Add to Cart
BHE13703927	KTE61712	96 Tx5	$2629		Add to Cart
BHE13703927	KTE61712	96 Tx50	$24569		Add to Cart

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