Matrices listed below were spiked with certain level of MEF2A and the recovery rates were calculated by comparing the measured value to the expected amount of MEF2A in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-101
94
EDTA plasma(n=5)
88-104
97
heparin plasma(n=5)
87-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MEF2A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-105%
92-104%
88-102%
EDTA plasma(n=5)
84-101%
84-101%
83-101%
heparin plasma(n=5)
80-100%
82-99%
87-96%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
MEF2A ELISA Kit allows for the in vitro quantitative determination of MEF2A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human MEF2A (Myocyte enhancer factor 2A) ELISA Kit