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Home / ELISA Kit

Human Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit

SKU : BHE13703882

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. Mediator of RNA polymerase II transcription subunit 14 encoded by MED14 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Mediator of RNA polymerase II transcription subunit 14 contains a bipartite nuclear localization signal. MED14 is known to escape chromosome X-inactivation.

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Mfr.No.	KTE61667
Description	This Human Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit employs a two-site sandwich ELISA to quantitate MED14.
Alternative Names	MED14; CRSP150; CRSP2; CSRP; CXorf4; DRIP150; EXLM1; MGC104513; RGR1; TRAP170; cofactor required for Sp1 transcriptional activation; subunit 2 (150kD); cofactor required for Sp1 transcriptional activation;
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Mediator of RNA polymerase II transcription subunit 14 microplate Human Mediator of RNA polymerase II transcription subunit 14 standard Human Mediator of RNA polymerase II transcription subunit 14 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit has high sensitivity and excellent specificity for detection of Human MED14. No significant cross-reactivity or interference between Human MED14 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. Mediator of RNA polymerase II transcription subunit 14 encoded by MED14 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Mediator of RNA polymerase II transcription subunit 14 contains a bipartite nuclear localization signal. MED14 is known to escape chromosome X-inactivation.
Notes	This Human Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit employs a two-site sandwich ELISA to quantitate MED14 in samples. An antibody specific for MED14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	9282
Antigen	MED14
Tag	MED14
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit employs a two-site sandwich ELISA to quantitate MED14 in samples. An antibody specific for MED14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703882	KTE61667	48 T	$339		Add to Cart
BHE13703882	KTE61667	96 T	$589		Add to Cart
BHE13703882	KTE61667	96 Tx5	$2629		Add to Cart
BHE13703882	KTE61667	96 Tx50	$24569		Add to Cart

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