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Home / ELISA Kit

Human Mediator of RNA polymerase II transcription subunit 13 (MED13) ELISA Kit

SKU : BHE13703884

MED13 encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of MED13 is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.

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Mfr.No.	KTE61669
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Mediator of RNA polymerase II transcription subunit 13 (MED13) ELISA Kit employs a two-site sandwich ELISA to quantitate MED13.
Alternative Names	MED13; ARC250; DRIP250; HSPC221; KIAA0593; THRAP1; TRAP240; activator-recruited cofactor 250 kDa component; mediator of RNA polymerase transcription; subunit 13 homolog; thyroid hormone receptor associate
Detection Method	Colorimetric
Kit Components	Human Mediator of RNA polymerase II transcription subunit 13 microplate Human Mediator of RNA polymerase II transcription subunit 13 standard Human Mediator of RNA polymerase II transcription subunit 13 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Mediator of RNA polymerase II transcription subunit 13 (MED13) ELISA Kit has high sensitivity and excellent specificity for detection of Human MED13. No significant cross-reactivity or interference between Human MED13 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MED13 encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of MED13 is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.
Notes	This Human Mediator of RNA polymerase II transcription subunit 13 (MED13) ELISA Kit employs a two-site sandwich ELISA to quantitate MED13 in samples. An antibody specific for MED13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	9969

Applications	ELISA
Applications Notes	This Human Mediator of RNA polymerase II transcription subunit 13 (MED13) ELISA Kit employs a two-site sandwich ELISA to quantitate MED13 in samples. An antibody specific for MED13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703884	KTE61669	48 T	$339		Add to Cart
BHE13703884	KTE61669	96 T	$589		Add to Cart
BHE13703884	KTE61669	96 Tx5	$2629		Add to Cart
BHE13703884	KTE61669	96 Tx50	$24569		Add to Cart

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