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Home / ELISA Kit

Human Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit

SKU : BHE13703886

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by MED12, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in MED12 cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome.

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Mfr.No.	KTE61671
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit employs a two-site sandwich ELISA to quantitate MED12.
Alternative Names	MED12; ARC240; CAGH45; FGS1; HOPA; KIAA0192; OKS; OPA1; TNRC11; TRAP230; CAG repeat protein 45; OPA-containing protein; activator-recruited cofactor 240 kDa component; human opposite paired; mediator of RNA po
Detection Method	Colorimetric
Kit Components	Human Mediator of RNA polymerase II transcription subunit 12 microplate Human Mediator of RNA polymerase II transcription subunit 12 standard Human Mediator of RNA polymerase II transcription subunit 12 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit has high sensitivity and excellent specificity for detection of Human MED12. No significant cross-reactivity or interference between Human MED12 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by MED12, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in MED12 cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome.
Notes	This Human Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit employs a two-site sandwich ELISA to quantitate MED12 in samples. An antibody specific for MED12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	9968

Applications	ELISA
Applications Notes	This Human Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit employs a two-site sandwich ELISA to quantitate MED12 in samples. An antibody specific for MED12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703886	KTE61671	48 T	$339		Add to Cart
BHE13703886	KTE61671	96 T	$589		Add to Cart
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BHE13703886	KTE61671	96 Tx50	$24569		Add to Cart

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