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Home / ELISA Kit

Human Mediator of RNA polymerase II transcription subunit 12-like protein (MED12L) ELISA Kit

SKU : BHE13703885

Mediator of RNA polymerase II transcription subunit 12-like protein encoded by MED12L is part of the Mediator complex, which is involved in transcriptional coactivation of nearly all RNA polymerase II-dependent genes. The Mediator complex links gene-specific transcriptional activators with the basal transcription machinery.

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Mfr.No.	KTE61670
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Mediator of RNA polymerase II transcription subunit 12-like protein (MED12L) ELISA Kit employs a two-site sandwich ELISA to quantitate MED12L.
Alternative Names	MED12L; FLJ46288; KIAA1635; NOPAR; TNRC11L; TRALP; TRALPUSH; mediator of RNA polymerase transcription; subunit 12 homolog (S. cerevisiae)-like; mediator of RNA polymerase transcription; subunit 12 homo
Detection Method	Colorimetric
Kit Components	Human Mediator of RNA polymerase II transcription subunit 12-like protein microplate Human Mediator of RNA polymerase II transcription subunit 12-like protein standard Human Mediator of RNA polymerase II transcription subunit 12-like protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Mediator of RNA polymerase II transcription subunit 12-like protein (MED12L) ELISA Kit has high sensitivity and excellent specificity for detection of Human MED12L. No significant cross-reactivity or interference between Human MED12L and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Mediator of RNA polymerase II transcription subunit 12-like protein encoded by MED12L is part of the Mediator complex, which is involved in transcriptional coactivation of nearly all RNA polymerase II-dependent genes. The Mediator complex links gene-specific transcriptional activators with the basal transcription machinery.
Notes	This Human Mediator of RNA polymerase II transcription subunit 12-like protein (MED12L) ELISA Kit employs a two-site sandwich ELISA to quantitate MED12L in samples. An antibody specific for MED12L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED12L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED12L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED12L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	116931

Applications	ELISA
Applications Notes	This Human Mediator of RNA polymerase II transcription subunit 12-like protein (MED12L) ELISA Kit employs a two-site sandwich ELISA to quantitate MED12L in samples. An antibody specific for MED12L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED12L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED12L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED12L bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703885	KTE61670	48 T	$339		Add to Cart
BHE13703885	KTE61670	96 T	$589		Add to Cart
BHE13703885	KTE61670	96 Tx5	$2629		Add to Cart
BHE13703885	KTE61670	96 Tx50	$24569		Add to Cart

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