Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of MED30 and the recovery rates were calculated by comparing the measured value to the expected amount of MED30 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
95
EDTA plasma(n=5)
86-104
95
heparin plasma(n=5)
87-99
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MED30 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-105%
89-105%
86-101%
EDTA plasma(n=5)
84-99%
82-99%
85-96%
heparin plasma(n=5)
82-94%
83-91%
81-98%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
Q96HR3
Applications
ELISA
Applications Notes
MED30 ELISA Kit allows for the in vitro quantitative determination of MED30 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human MED30 (Mediator complex subunit 30) ELISA Kit