Human MDM2(E3 ubiquitin-protein ligase Mdm2) ELISA Kit

Mfr.No.

EH10021

Description

Human MDM2(E3 ubiquitin-protein ligase Mdm2) ELISA Kit

Alternative Names

E3 ubiquitin-protein ligase Mdm2 ELISA Kit, Double minute 2 protein ELISA Kit, Hdm2 ELISA Kit, Oncoprotein Mdm2 ELISA Kit, RING-type E3 ubiquitin transferase Mdm2 ELISA Kit, p53-binding protein Mdm2 ELISA Kit, MDM2 ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

0.313-20ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of MDM2 and the recovery rates were calculated by comparing the measured value to the expected amount of MDM2 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	95-105	99
EDTA plasma(n=5)	95-105	99
heparin plasma(n=5)	96-103	100

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MDM2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	90-103%	89-99%	90-104%
EDTA plasma(n=5)	89-99%	89-95%	89-100%
heparin plasma(n=5)	90-99%	90-97%	92-100%

Sensitivity

< 0.188ng/ml

Notes

For Research Use Only

UniProt ID	Q00987

Applications	ELISA
Applications Notes	MDM2 ELISA Kit allows for the in vitro quantitative determination of MDM2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10808544	EH10021	96T	$400		Add to Cart

Human MDM2(E3 ubiquitin-protein ligase Mdm2) ELISA Kit

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