Matrices listed below were spiked with certain level of MC4R and the recovery rates were calculated by comparing the measured value to the expected amount of MC4R in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-98
94
EDTA plasma(n=5)
86-102
94
heparin plasma(n=5)
90-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MC4R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
MC4R ELISA Kit allows for the in vitro quantitative determination of MC4R concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human MC4R(Melanocortin receptor 4) ELISA Kit