Human Matrix Metalloproteinase 9

Mfr.No.

E0936Hu

Description

This sandwich kit is for the accurate quantitative detection of human Matrix Metalloproteinase 9 (also known as MMP-9) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

67 kDa Matrix Metalloproteinase-9;82 kDa Matrix Metalloproteinase-9);92 kDa gelatinase;92 kDa type IV collagenase;CLG4B;Gelatinase B;GELB;MANDP2;Matrix Metalloproteinase-9;MMP-9

Reactivity

Human

Detection Range

30-9000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

78458

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

15.12ng/L

Background

Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed:2551898, PubMed:1480034, PubMed:12879005). Could play a role in bone osteoclastic resorption (By similarity). Cleaves KiSS1 at a Gly-|-Leu bond (PubMed:12879005). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed:32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed:1480034). Degrades fibronectin but not laminin or Pz-peptide.

Gene ID	4318
UniProt ID	P14780
Antigen	MMP-9

Research Area

Cancer; Cell Biology

Protocol

These standard curves of E0936Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.117	2.236	2.179
	2.355
2400ng/L	1.285	1.416	1.359
	1.547
1200ng/L	0.727	0.771	0.714
	0.814
600ng/L	0.54	0.505	0.448
	0.471
300ng/L	0.22	0.243	0.186
	0.267
0ng/L	0.042	0.057	0
	0.071

Precision

We measured random samples of E0936Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1563.3	101.33	6.48
Intra-Assay	2	18	3312.33	110.21	3.33
Intra-Assay	3	18	497.72	25.3	5.08

Storage	2-8°C

Reference	Association between Serum Vitamin D Concentration Status and Matrix Metalloproteinase-9 in Patients Undergoing Elective Percutaneous Coronary Intervention Journal: Iran J Pharm Res. 2020 Fall;19(4):135-142 Serum galectin-3BP as a novel marker of obesity and metabolic syndrome in Chinese adolescents Journal: BMJ Open Diabetes Res Care. 2021 Apr;9(1):e001894. Probiotic Soy Milk Consumption and Renal Function Among Type 2 Diabetic Patients with Nephropathy: a Randomized Controlled Clinical Trial Journal: Probiotics Antimicrob Proteins. 2019 Mar;11(1):124-132.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102611	E0936Hu	48T	$320		Add to Cart
BHE12102611	E0936Hu	96T	$458		Add to Cart

Human Matrix Metalloproteinase 9 - MMP-9 ELISA Kit

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