Human matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA Kit

Mfr.No.

CSB-E08006h

Alternative Names

matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase), CLG4B, GELB, MANDP2, MMP-9, macrophage gelatinase, matrix metalloproteinase 9, matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase), type V collagenase

Reactivity

Human

Detection Range

0.312 ng/mL-20 ng/mL

Sample Type

Serum, Cell culture supernatants, Plasma, Urine, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human C3b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-98
EDTA plasma (n=4)	97	91-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C3b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

82-92

1:2

Average %

Range %

92-103

1:4

Average %

Range %

93-103

1:8

Average %

Range %

89-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.115	2.003	2.059	1.932
100	1.748	1.736	1.742	1.615
50	1.245	1.266	1.256	1.129
25	0.756	0.782	0.769	0.642
12.5	0.568	0.572	0.570	0.443
6.25	0.333	0.342	0.338	0.211
3.12	0.199	0.187	0.193	0.066
0	0.128	0.126	0.127	?

Species

Homo sapiens

Sensitivity

0.284 ng/mL

Limit Of Detection

450 nm

UniProt ID	P14780
Antigen	matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

Applications

ELISA

Research Area

MMP9

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503306	CSB-E08006h	96T	$495	Add to Cart
BHE10503306	CSB-E08006h	5x96T	$2376	Add to Cart
BHE10503306	CSB-E08006h	10x96T	$4702.50	Add to Cart

Human matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA Kit

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