Human Matrix metalloproteinase 8/Neutrophil collagenase,MMP-8 ELISA kit

Mfr.No.

CSB-E04680h

Sensitivity

9.77 pg/mL

Detection Range

39.06 pg/mL-2500 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

matrix metallopeptidase 8 (neutrophil collagenase), CLG1, HNC, PMNL-CL, PMNL collagenase, matrix metalloproteinase 8, matrix metalloproteinase 8 (neutrophil collagenase)

Recovery

The recovery of fish trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-100
EDTA plasma (n=4)	102	96-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	101
	Range %	95-105
1:40	Average %	91
	Range %	84-95
1:80	Average %	106
	Range %	100-111
1:160	Average %	104
	Range %	97-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
20	0.231	0.227	0.229
10	0.342	0.337	0.340
5	0.497	0.482	0.490
2.5	0.663	0.645	0.654
1.25	0.932	0.918	0.925
0.625	1.300	1.246	1.273
0.312	1.774	1.724	1.749
0	2.434	2.389	2.412

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P22894

Applications	ELISA
Research Area	MMP8

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503305	CSB-E04680h	96T	$395	Add to Cart
BHE10503305	CSB-E04680h	5x96T	$1896	Add to Cart
BHE10503305	CSB-E04680h	10x96T	$3752.50	Add to Cart

Human Matrix metalloproteinase 8/Neutrophil collagenase,MMP-8 ELISA kit

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