Human Matrix metalloproteinase-28(MMP28) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL014675HU

Alternative Names

matrix metallopeptidase 28, EPILYSIN, MM28, MMP-28, MMP25, matrix metalloproteinase 28

Reactivity

Human

Detection Range

0.625 ng/mL-40 ng/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of bovine INHBA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-100
EDTA plasma (n=4)	96	91-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine INHBA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-98
1:2	Average %	94
	Range %	86-103
1:4	Average %	92
	Range %	89-98
1:8	Average %	96
	Range %	91-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.156 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q9H239
Antigen	matrix metallopeptidase 28

Applications

ELISA

Research Area

MMP28

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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