Human Matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit

Mfr.No.

CSB-E04675h

Alternative Names

matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), CLG4, CLG4A, MMP-II, MONA, TBE-1, collagenase type IV-A, matrix metalloproteinase 2, matrix metalloproteinase-II, neutrophil gelatinase

Reactivity

Human

Detection Range

0.78 ng/mL-50 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	87	82-91

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	101
	Range %	96-107
1:4	Average %	92
	Range %	88-96
1:8	Average %	89
	Range %	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.651	2.529	2.590	2.382
2000	2.004	1.903	1.954	1.746
1000	1.310	1.385	1.348	1.140
500	0.829	0.851	0.840	0.632
250	0.492	0.476	0.484	0.276
125	0.347	0.337	0.342	0.134
62.5	0.285	0.294	0.290	0.082
0	0.203	0.212	0.208	?

Species

Homo sapiens

Sensitivity

0.195 ng/mL

Limit Of Detection

450 nm

UniProt ID	P08253
Antigen	matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

Applications

ELISA

Research Area

MMP2

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503301	CSB-E04675h	96T	$615	Add to Cart
BHE10503301	CSB-E04675h	5x96T	$2952	Add to Cart
BHE10503301	CSB-E04675h	10x96T	$5842.50	Add to Cart

Human Matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit

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