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Home / ELISA Kit

Human Matrix Metalloproteinase 12 (MMP-12) ELISA Kit

SKU : BHE13703814

MMP-12 encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. MMP-12 may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). MMP-12 is part of a cluster of MMP genes on chromosome 11.

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Mfr.No.	KTE61599
Reactivity	Human
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Matrix Metalloproteinase 12 (MMP-12) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP12.
Alternative Names	MMP12; HME; MGC138506; MME; macrophage elastase; macrophage metalloelastase; matrix metalloproteinase 12; matrix metalloproteinase 12 (macrophage elastase)
Detection Method	Colorimetric
Kit Components	Human Matrix Metalloproteinase 12 microplate Human Matrix Metalloproteinase 12 standard Human Matrix Metalloproteinase 12 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Matrix Metalloproteinase 12 (MMP-12) ELISA Kit has high sensitivity and excellent specificity for detection of Human MMP12. No significant cross-reactivity or interference between Human MMP12 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MMP-12 encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. MMP-12 may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). MMP-12 is part of a cluster of MMP genes on chromosome 11.
Notes	This Human Matrix Metalloproteinase 12 (MMP-12) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP12 in samples. An antibody specific for MMP12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4321
Antigen	MMP12

Applications	ELISA
Applications Notes	This Human Matrix Metalloproteinase 12 (MMP-12) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP12 in samples. An antibody specific for MMP12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703814	KTE61599	48 T	$359		Add to Cart
BHE13703814	KTE61599	96 T	$619		Add to Cart
BHE13703814	KTE61599	96 Tx5	$2809		Add to Cart
BHE13703814	KTE61599	96 Tx50	$26209		Add to Cart

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