Human Matrix Metalloproteinase 12(MMP-12) ELISA Kit

Human Matrix Metalloproteinase 12(MMP-12) ELISA Kit

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Mfr.No.

CSB-E13208h

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

matrix metallopeptidase 12 (macrophage elastase), HME, MGC138506, MME, macrophage elastase, macrophage metalloelastase, matrix metalloproteinase 12, matrix metalloproteinase 12 (macrophage elastase)

Recovery

The recovery of human TAGLN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-90
EDTA plasma (n=4)	99	95-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TAGLN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-93
1:2	Average %	101
	Range %	95-105
1:4	Average %	97
	Range %	92-101
1:8	Average %	88
	Range %	83-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.823	2.902	2.863	2.754
10	2.166	2.355	2.261	2.152
5	1.443	1.496	1.470	1.361
2.5	1.035	1.052	1.044	0.935
1.25	0.558	0.596	0.577	0.468
0.625	0.374	0.375	0.375	0.266
0.312	0.231	0.248	0.240	0.131
0	0.108	0.109	0.109

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P39900

Applications	ELISA
Research Area	MMP12

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10503288	CSB-E13208h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.