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Home / ELISA Kit

Human Matrix Metalloproteinase 1 (MMP1) ELISA Kit

SKU : BHE13703338

MMP-1 is produced by fibroblasts, chondrocytes, macrophages, endothelial cells, and osteoblasts. It is induced by the pro-inflammatory cytokines IL-1 and TNF-alpha, various growth factors such as EGF, PDGF, FGF basic, and Oncostatin M, chemical agents such as cAMP and phorbol esters and events occurring at the cell surface such as cell fusion and phagocytosis. MMP-1 plays a significant role in the degradation of different types of collagen in extracellular matrix remodeling. It is implicated in a variety of processes involving collagen degradation such as emphysema, atherosclerosis, rheumatoid and osteoarthritis, periodontal and respiratory disease, angiogenesis and tumorigenesis, tissue remodeling and wound healing, and inflammatory bowel disease.

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Mfr.No.	KTE61122
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Matrix Metalloproteinase 1 (MMP1) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP1.
Alternative Names	MMP1; CLGN; CLG1; Collagenase; Interstitial Collagenase; Vertebrate Collagenase; Fibroblast Collagenase
Detection Method	Colorimetric
Kit Components	Human Matrix Metalloproteinase 1 microplate Human Matrix Metalloproteinase 1 standard Human Matrix Metalloproteinase 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Matrix Metalloproteinase 1 (MMP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MMP1. No significant cross-reactivity or interference between Human MMP1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MMP-1 is produced by fibroblasts, chondrocytes, macrophages, endothelial cells, and osteoblasts. It is induced by the pro-inflammatory cytokines IL-1 and TNF-alpha, various growth factors such as EGF, PDGF, FGF basic, and Oncostatin M, chemical agents such as cAMP and phorbol esters and events occurring at the cell surface such as cell fusion and phagocytosis. MMP-1 plays a significant role in the degradation of different types of collagen in extracellular matrix remodeling. It is implicated in a variety of processes involving collagen degradation such as emphysema, atherosclerosis, rheumatoid and osteoarthritis, periodontal and respiratory disease, angiogenesis and tumorigenesis, tissue remodeling and wound healing, and inflammatory bowel disease.
Notes	This Human Matrix Metalloproteinase 1 (MMP1) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP1 in samples. An antibody specific for MMP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4312

Applications	ELISA
Applications Notes	This Human Matrix Metalloproteinase 1 (MMP1) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP1 in samples. An antibody specific for MMP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703338	KTE61122	48 T	$359		Add to Cart
BHE13703338	KTE61122	96 T	$619		Add to Cart
BHE13703338	KTE61122	96 Tx5	$2809		Add to Cart
BHE13703338	KTE61122	96 Tx50	$26209		Add to Cart

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