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Home / ELISA Kit

Human Matrix extracellular phosphoglycoprotein (MEPE) ELISA Kit

SKU : BHE13703865

MEPE encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants.

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Mfr.No.	KTE61650
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Matrix extracellular phosphoglycoprotein (MEPE) ELISA Kit employs a two-site sandwich ELISA to quantitate MEPE.
Alternative Names	MEPE; OF45; matrix; extracellular phosphoglycoprotein with ASARM motif (bone)
Detection Method	Colorimetric
Kit Components	Human Matrix extracellular phosphoglycoprotein microplate Human Matrix extracellular phosphoglycoprotein standard Human Matrix extracellular phosphoglycoprotein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Matrix extracellular phosphoglycoprotein (MEPE) ELISA Kit has high sensitivity and excellent specificity for detection of Human MEPE. No significant cross-reactivity or interference between Human MEPE and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MEPE encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants.
Notes	This Human Matrix extracellular phosphoglycoprotein (MEPE) ELISA Kit employs a two-site sandwich ELISA to quantitate MEPE in samples. An antibody specific for MEPE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMEPE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEPE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEPE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	56955

Applications	ELISA
Applications Notes	This Human Matrix extracellular phosphoglycoprotein (MEPE) ELISA Kit employs a two-site sandwich ELISA to quantitate MEPE in samples. An antibody specific for MEPE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMEPE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEPE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEPE bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703865	KTE61650	48 T	$359		Add to Cart
BHE13703865	KTE61650	96 T	$619		Add to Cart
BHE13703865	KTE61650	96 Tx5	$2809		Add to Cart
BHE13703865	KTE61650	96 Tx50	$26209		Add to Cart

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