Human Matrix Extracellular Phosphoglycoprotein

Mfr.No.

E1485Hu

Description

This sandwich kit is for the accurate quantitative detection of human Matrix Extracellular Phosphoglycoprotein (also known as MEPE) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Matrix extracellular phosphoglycoprotein;MEPE;OF45;Osteoblast/osteocyte factor 45;Osteoregulin

Reactivity

Human

Detection Range

10-2000ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

58419

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.26ng/ml

Background

Promotes renal phosphate excretion and inhibits intestinal phosphate absorption (PubMed: 14962809, PubMed: 19005008). Promotes bone mineralization by osteoblasts and cartilage mineralization by chondrocytes (PubMed: 18162525, PubMed: 19998030, PubMed: 22766095). Regulates the mineralization of the extracellular matrix of the craniofacial complex, such as teeth, bone and cartilage (By similarity). Promotes dental pulp stem cell proliferation and differentiation (PubMed: 22341070).

Gene ID	56955
UniProt ID	Q9NQ76
Antigen	MEPE

Research Area

Signal Transduction

Protocol

These standard curves of E1485Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/ml	2.599	2.602	2.564
	2.604
600ng/ml	1.516	1.534	1.497
	1.553
300ng/ml	0.798	0.844	0.807
	0.891
150ng/ml	0.475	0.471	0.433
	0.467
75ng/ml	0.188	0.202	0.165
	0.216
0ng/ml	0.04	0.038	0
	0.035

Precision

We measured random samples of E1485Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	139.3	6.53	4.7
Intra-Assay	2	18	352.4	18.16	5.2
Intra-Assay	3	18	733.8	19	2.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103102	E1485Hu	48T	$320		Add to Cart
BHE12103102	E1485Hu	96T	$458		Add to Cart

Human Matrix Extracellular Phosphoglycoprotein - MEPE ELISA Kit

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