Human Matrilysin - MAT ELISA Kit

Mfr.No.

E1735Hu

Description

This sandwich kit is for the accurate quantitative detection of human Matrilysin (also known as MAT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

MAT;Matrilysin;Matrin;Matrix Metalloproteinase-7;MMP-7;MPSL1;PUMP-1;Pump-1 protease;Uterine metalloproteinase;

Reactivity

Human

Detection Range

0.05-15ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

29677

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.023ng/ml

Background

Matrilysin also known as matrix metalloproteinase-7 (MMP-7), pump-1 protease (PUMP-1), or uterine metalloproteinase is an enzyme in humans that is encoded by the MMP7 gene.(5) The enzyme (EC 3.4.24.23) has also been known as matrin, putative (or punctuated) metalloproteinase-1, matrix metalloproteinase pump 1, PUMP-1 proteinase, PUMP, metalloproteinase pump-1, putative metalloproteinase, MMP). Human MMP-7 has a molecular weight around 30 kDa.

Gene ID	4316
UniProt ID	P09237
Antigen	MAT

Research Area

Immunology

Protocol

These standard curves of E1735Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.347	2.285	2.232
	2.224
4ng/ml	1.845	1.878	1.825
	1.911
2ng/ml	1.106	1.101	1.048
	1.097
1ng/ml	0.636	0.62	0.567
	0.604
0.5ng/ml	0.348	0.357	0.304
	0.367
0ng/ml	0.041	0.053	0
	0.065

Precision

We measured random samples of E1735Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.34	0.06	4.32
Intra-Assay	2	18	2.8	0.13	4.49
Intra-Assay	3	18	0.85	0.05	5.71

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103317	E1735Hu	48T	$320		Add to Cart
BHE12103317	E1735Hu	96T	$458		Add to Cart

Human Matrilysin - MAT ELISA Kit

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