Human Mast Cell Carboxypeptidase ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13756h

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

carboxypeptidase A3 (mast cell), MC-CPA, carboxypeptidase A3

Recovery

The recovery of human EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	80-94
EDTA plasma (n=4)	98	92-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	95
	Range %	90-99
1:4	Average %	90
	Range %	85-96
1:8	Average %	90
	Range %	84-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P15088

Applications	ELISA
Research Area	CPA3

Storage	Store at 2-8°C. Please refer to protocol.

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