Matrices listed below were spiked with certain level of MAS-R and the recovery rates were calculated by comparing the measured value to the expected amount of MAS-R in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-104
97
EDTA plasma(n=5)
86-103
94
heparin plasma(n=5)
89-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MAS-R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-105%
88-102%
88-94%
EDTA plasma(n=5)
84-101%
82-96%
82-97%
heparin plasma(n=5)
81-93%
80-89%
84-91%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P35410
Applications
ELISA
Applications Notes
MAS-RELISA Kit allows for the in vitro quantitative determination of MAS-R concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human MAS-R(Mas-related G-protein coupled receptor MRG)ELISA Kit