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Home / ELISA Kit

Human MARVEL domain-containing protein 2 (MARVELD2) ELISA Kit

SKU : BHE13703936

MARVELD2, or tricellulin (TRIC), is an integral membrane protein concentrated at the vertically oriented TJ strands of tricellular contacts.The 555-amino acid protein contains cytoplasmic N and C termini, 2 transmembrane domains, and 2 extracellular loops. Northern blot analysis detected high expression of Tric in epithelium-derived tissues, including small intestine, kidney, and lung. Western blot analysis of mouse epithelial cell lines revealed an approximately 66-kD protein, as well as larger phosphorylated intracellular forms. Immunofluorescence microscopy showed expression of Tric predominantly at tricellular contacts where occludin (OCLN)-positive bicellular TJs converged. Tric-positive structures appeared as short rods through confocal microscopy.

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Mfr.No.	KTE61721
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human MARVEL domain-containing protein 2 (MARVELD2) ELISA Kit employs a two-site sandwich ELISA to quantitate MARVELD2.
Alternative Names	MARVELD2; DFNB49; FLJ30532; MARVD2; MRVLDC2; Tric; MARVEL (membrane-associating) domain containing 2; tricellulin
Detection Method	Colorimetric
Kit Components	Human MARVEL domain-containing protein 2 microplate Human MARVEL domain-containing protein 2 standard Human MARVEL domain-containing protein 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human MARVEL domain-containing protein 2 (MARVELD2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MARVELD2. No significant cross-reactivity or interference between Human MARVELD2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MARVELD2, or tricellulin (TRIC), is an integral membrane protein concentrated at the vertically oriented TJ strands of tricellular contacts.The 555-amino acid protein contains cytoplasmic N and C termini, 2 transmembrane domains, and 2 extracellular loops. Northern blot analysis detected high expression of Tric in epithelium-derived tissues, including small intestine, kidney, and lung. Western blot analysis of mouse epithelial cell lines revealed an approximately 66-kD protein, as well as larger phosphorylated intracellular forms. Immunofluorescence microscopy showed expression of Tric predominantly at tricellular contacts where occludin (OCLN)-positive bicellular TJs converged. Tric-positive structures appeared as short rods through confocal microscopy.
Notes	This Human MARVEL domain-containing protein 2 (MARVELD2) ELISA Kit employs a two-site sandwich ELISA to quantitate MARVELD2 in samples. An antibody specific for MARVELD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMARVELD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MARVELD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MARVELD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	153562

Applications	ELISA
Applications Notes	This Human MARVEL domain-containing protein 2 (MARVELD2) ELISA Kit employs a two-site sandwich ELISA to quantitate MARVELD2 in samples. An antibody specific for MARVELD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMARVELD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MARVELD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MARVELD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703936	KTE61721	48 T	$339		Add to Cart
BHE13703936	KTE61721	96 T	$589		Add to Cart
BHE13703936	KTE61721	96 Tx5	$2629		Add to Cart
BHE13703936	KTE61721	96 Tx50	$24569		Add to Cart

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