Human Marapsin/Pancresin ELISA Kit PicoKine®

Mfr.No.

EK1479

Sensitivity

<10 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.029	0.06	0.124	0.195	0.432	0.834	1.369	1.897

Description

Human Marapsin/Pancresin ELISA Kit PicoKine® (96 Tests). Quantitate Human PRSS27 in cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Serine protease 27;3.4.21.-;Marapsin;Pancreasin;PRSS27;MPN;UNQ1884/PRO4327;

Molecular Weight

31940 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: M35-K290

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Marapsin

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	167	13161	5662	161	1417	5245
Standard deviation	10.35	58.59	311.41	12.39	77.93	326.2%9
CV (%)	6.2%	4.2%	5.5%	7.7%	5.5%	6.2%

Background

Pancreasin, also known as PRSS27 or Marapsin, is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. It belongs to the group-1 subfamily of serine proteases. The pancreasin gene is mapped to chromosome 16p13.3. Pancreasin is resistant to inactivation by large molecular mass trypsin inhibitors, but it is sensitive to benzamidine and leupeptin.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	167	176	171	165	169	4.2	2.4%
Sample 2	1395	1278	1352	1276	1325	50.59	3.8%
Sample 3	5662	5287	5308	6068	5581	318.07	5.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	83886
Gene Name	PRSS27
UniProt ID	Q9BQR3
Protein Name	Serine protease 27
Tissue Specificity	Expressed predominantly in the pancreas. .

Applications	ELISA
Research Area	Cell Biology,Proteolysis/Ubiquitin,Proteolytic Enzymes

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001075	EK1479	96 wells/kit	$499		Add to Cart

Human Marapsin/Pancresin ELISA Kit PicoKine®

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