Matrices listed below were spiked with certain level of MAPK and the recovery rates were calculated by comparing the measured value to the expected amount of MAPK in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-105
98
EDTA plasma(n=5)
93-104
96
heparin plasma(n=5)
86-99
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MAPK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
MAPK ELISA Kit allows for the in vitro quantitative determination of MAPK concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human MAPK(Mitogen Activated Protein Kinase) ELISA Kit