Human MAP1LC3B(Microtubule-associated proteins 1A/1B light chain 3B) ELISA Kit

Mfr.No.

ELK8620

Sensitivity

0.059 ng/mL

Detection Range

0.16-10 ng/mL

Reactivity

Human

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

LC3B; MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP

Recovery

Matrices listed below were spiked with certain level of recombinant MAP1LC3B and the recovery rates were calculated by comparing the measured value to the expected amount of MAP1LC3B in samples.

Matrix	Recovery range	Average
serum(n=5)	88-101%	94%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	78-91%	84%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MAP1LC3B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	84-99%	83-101%	86-99%	93-101%
EDTA plasma(n=5)	88-95%	98-107%	90-99%	88-97%
Heparin plasma(n=5)	87-93%	91-103%	82-99%	99-104%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
10.00	2.075	1.990
5.00	1.724	1.639
2.50	1.132	1.047
1.25	0.865	0.780
0.63	0.597	0.512
0.32	0.372	0.287
0.16	0.215	0.130
0.00	0.085	0.000

Limit Of Detection

10 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAP1LC3B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAP1LC3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAP1LC3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAP1LC3B in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	A6NCE7

Applications	ELISA
Research Area	Metabolic pathway

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200907	ELK8620	48T	$265		Add to Cart
BHE15200907	ELK8620	96T	$376		Add to Cart

Human MAP1LC3B(Microtubule-associated proteins 1A/1B light chain 3B) ELISA Kit

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