JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / ELISA Kit

Human Mannma binding protein/mannan binding lectin (MBP/MBL) ELISA Kit

SKU : BHE13703917

Mannosebinding protein (MBP), initiates the lectin branch of the innate immune response by binding to the surface of potentially pathogenic microorganisms and initiating complement fixation through an N-terminal collagen-like domain. MBL is a key component in immune response in that it can directly trigger neutralization of invading microorganisms by an Ab-independent mechanism.It binds to sugars on the surface of bacterial, fungal and parasitic cells through C-terminal, Ca2 -dependent carbohydrate-recognition domains. Mutations of human MBL are associated with immunodeficiency resulting from a reduction in the ability of the mutant MBL to initiate complement fixation.

Continuing the saving: $50 Off All ELISA Kits Offer Extended!

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Abbkine Scientific Co., Ltd.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	KTE61702
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Mannma binding protein/mannan binding lectin (MBP/MBL) ELISA Kit employs a two-site sandwich ELISA to quantitate MBL2.
Alternative Names	MBL2; COLEC1; HSMBPC; MBL; MBP; MBP1; MGC116832; MGC116833; mannan-binding lectin; mannose-binding lectin 2; soluble (opsonic defect); mannose-binding protein C; soluble mannose-binding lectin
Detection Method	Colorimetric
Kit Components	Human Mannma binding protein/mannan binding lectin microplate Human Mannma binding protein/mannan binding lectin standard Human Mannma binding protein/mannan binding lectin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Mannma binding protein/mannan binding lectin (MBP/MBL) ELISA Kit has high sensitivity and excellent specificity for detection of Human MBL2. No significant cross-reactivity or interference between Human MBL2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Mannosebinding protein (MBP), initiates the lectin branch of the innate immune response by binding to the surface of potentially pathogenic microorganisms and initiating complement fixation through an N-terminal collagen-like domain. MBL is a key component in immune response in that it can directly trigger neutralization of invading microorganisms by an Ab-independent mechanism.It binds to sugars on the surface of bacterial, fungal and parasitic cells through C-terminal, Ca2 -dependent carbohydrate-recognition domains. Mutations of human MBL are associated with immunodeficiency resulting from a reduction in the ability of the mutant MBL to initiate complement fixation.
Notes	This Human Mannma binding protein/mannan binding lectin (MBP/MBL) ELISA Kit employs a two-site sandwich ELISA to quantitate MBL2 in samples. An antibody specific for MBL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMBL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4153

Applications	ELISA
Applications Notes	This Human Mannma binding protein/mannan binding lectin (MBP/MBL) ELISA Kit employs a two-site sandwich ELISA to quantitate MBL2 in samples. An antibody specific for MBL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMBL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

No Description Available.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13703917	KTE61702	48 T	$359		Add to Cart
BHE13703917	KTE61702	96 T	$619		Add to Cart
BHE13703917	KTE61702	96 Tx5	$2809		Add to Cart
BHE13703917	KTE61702	96 Tx50	$26209		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.