Human mannan-binding lectin serine peptidase 2 (MASP2)ELISA kit

Mfr.No.

CSB-E17966h

Sensitivity

5.86 pg/mL

Detection Range

23.44 pg/mL-1500 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

mannan-binding lectin serine peptidase 2, MAP19, MASP-2, sMAP, MBL-associated plasma protein of 19 kD, MBL-associated serine protease 2, mannan-binding lectin serine protease 2, small MBL-associated protein

Recovery

The recovery of human GM-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-100
EDTA plasma (n=4)	96	90-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GM-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-99
1:2	Average %	95
	Range %	88-102
1:4	Average %	95
	Range %	86-99
1:8	Average %	95
	Range %	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.987	2.913	2.950	2.792
500	2.417	2.429	2.423	2.265
250	1.674	1.686	1.680	1.522
125	0.954	0.963	0.959	0.801
62.5	0.576	0.562	0.569	0.411
31.2	0.386	0.394	0.390	0.232
15.6	0.261	0.268	0.265	0.107
0	0.156	0.159	0.158	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	O00187

Applications	ELISA
Research Area	MASP2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503272	CSB-E17966h	96T	$790	Add to Cart
BHE10503272	CSB-E17966h	5x96T	$3792	Add to Cart
BHE10503272	CSB-E17966h	10x96T	$7505	Add to Cart

Human mannan-binding lectin serine peptidase 2 (MASP2)ELISA kit

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