Human Mannan-binding Lectin Serine Peptidase 1

Mfr.No.

E5632Hu

Sensitivity

0.58ng/mL

Detection Range

1-400ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Complement factor MASP-3;Complement-activating component of Ra-reactive factor;Mannan-binding lectin serine protease 1;Mannan-binding lectin serine protease 1 heavy chain;Mannan-binding lectin serine protease 1 light chain;Mannose-binding lectin-associated serine protease 1;Mannose-binding protein-associated serine protease;MASP 1;MASP1;MASP-1;Ra-reactive factor serine protease p100;RaRF;Serine protease 5;

Molecular Weight

79247

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5632Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	23.6	1.76	7.4
Intra-Assay	2	18	53.5	3.47	6.5
Intra-Assay	3	18	84.9	4.21	5

Background

Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5. Also plays a role in development (PubMed: 21258343).

Protocol

These standard curves of E5632Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/mL	2.271	2.313	2.275
	2.356
120ng/mL	1.75	1.821	1.783
	1.892
60ng/mL	1.151	1.233	1.194
	1.314
30ng/mL	0.667	0.693	0.654
	0.719
15ng/mL	0.443	0.429	0.39
	0.414
0ng/mL	0.045	0.039	0
	0.033

Gene ID	5648
UniProt ID	P48740

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106994	E5632Hu	48T	$320		Add to Cart
BHE12106994	E5632Hu	96T	$458		Add to Cart

Human Mannan-binding Lectin Serine Peptidase 1 - MASP1 ELISA Kit

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