Human mammalian target of rapamyein,mTOR ELISA Kit

Mfr.No.

CSB-E09038h

Alternative Names

mammalian target of rapamyein,mTOR

Reactivity

Human

Detection Range

1.56 pg/mL-100 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse CTLA-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	96-108
EDTA plasma (n=4)	87	83-90

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTLA-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	95-99
1:2	Average %	102
	Range %	97-106
1:4	Average %	86
	Range %	83-89
1:8	Average %	94
	Range %	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.39 pg/mL

Limit Of Detection

450 nm

UniProt ID	P42345
Antigen	mammalian target of rapamyein,mTOR

Applications

ELISA

Research Area

mTOR

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503269	CSB-E09038h	96T	$790	Add to Cart
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BHE10503269	CSB-E09038h	10x96T	$7505	Add to Cart