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Home / ELISA Kit

Human Mammaglobin-A (SCGB2A2) ELISA Kit

SKU : BHE13702955

SCGB2A2 is member of the superfamily of secretoglobins, a group of small dimeric secreted and sometimes glycosylated proteins. Expressed mainly in mucosa, secretoglobins seems to be involved in cell signalling, immune response, chemotaxis, and could also serve as transporters for steroid hormones in humans. MGB1 encodes a deduced 93-amino acid protein with a 19-amino acid hydrophobic peptide signal sequence and a predicted molecular mass of 10.5-kD. The protein shares 42% sequence identity with the rat prostatein protein (rPSC3). It also shares high homology with rabbit and human uteroglobin (UGB) genes, including the conservation of cysteines known to play a role in disulfide bond formation between uteroglobin subunits and a conserved tyrosine required for progesterone binding to the uteroglobin dimer.

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Mfr.No.	KTE60739
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Mammaglobin-A (SCGB2A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SCGB2A2.
Alternative Names	SCGB2A2; MGB1; MGC71974; UGB2; mammaglobin 1; mammaglobin A
Detection Method	Colorimetric
Kit Components	Human Mammaglobin-A microplate Human Mammaglobin-A standard Human Mammaglobin-A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Mammaglobin-A (SCGB2A2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCGB2A2. No significant cross-reactivity or interference between Human SCGB2A2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SCGB2A2 is member of the superfamily of secretoglobins, a group of small dimeric secreted and sometimes glycosylated proteins. Expressed mainly in mucosa, secretoglobins seems to be involved in cell signalling, immune response, chemotaxis, and could also serve as transporters for steroid hormones in humans. MGB1 encodes a deduced 93-amino acid protein with a 19-amino acid hydrophobic peptide signal sequence and a predicted molecular mass of 10.5-kD. The protein shares 42% sequence identity with the rat prostatein protein (rPSC3). It also shares high homology with rabbit and human uteroglobin (UGB) genes, including the conservation of cysteines known to play a role in disulfide bond formation between uteroglobin subunits and a conserved tyrosine required for progesterone binding to the uteroglobin dimer.
Notes	This Human Mammaglobin-A (SCGB2A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SCGB2A2 in samples. An antibody specific for SCGB2A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCGB2A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCGB2A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCGB2A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4250

Applications	ELISA
Applications Notes	This Human Mammaglobin-A (SCGB2A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SCGB2A2 in samples. An antibody specific for SCGB2A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCGB2A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCGB2A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCGB2A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702955	KTE60739	48 T	$339		Add to Cart
BHE13702955	KTE60739	96 T	$589		Add to Cart
BHE13702955	KTE60739	96 Tx5	$2629		Add to Cart
BHE13702955	KTE60739	96 Tx50	$24569		Add to Cart

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