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Home / ELISA Kit

Human MAGUK p55 subfamily member 4 (MPP4) ELISA Kit

SKU : BHE13703773

MPP4 encodes a member of the membrane-associated guanylate kinase (MAGUK) protein family, with an N-terminal PDZ domain, a central src homology 3 region (SH3), and a C-terminal guanylate kinase-like (GUK) domain. The protein is localized to the outer limiting membrane in the retina, and is thought to function in photoreceptor polarity and the organization of specialized intercellular junctions. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

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Mfr.No.	KTE61558
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human MAGUK p55 subfamily member 4 (MPP4) ELISA Kit employs a two-site sandwich ELISA to quantitate MPP4.
Alternative Names	MPP4; ALS2CR5; DLG6; amyotrophic lateral sclerosis 2 chromosomal region candidate gene 5 protein; discs; large homolog 6; membrane protein; palmitoylated 4
Detection Method	Colorimetric
Kit Components	Human MAGUK p55 subfamily member 4 microplate Human MAGUK p55 subfamily member 4 standard Human MAGUK p55 subfamily member 4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human MAGUK p55 subfamily member 4 (MPP4) ELISA Kit has high sensitivity and excellent specificity for detection of Human MPP4. No significant cross-reactivity or interference between Human MPP4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MPP4 encodes a member of the membrane-associated guanylate kinase (MAGUK) protein family, with an N-terminal PDZ domain, a central src homology 3 region (SH3), and a C-terminal guanylate kinase-like (GUK) domain. The protein is localized to the outer limiting membrane in the retina, and is thought to function in photoreceptor polarity and the organization of specialized intercellular junctions. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Notes	This Human MAGUK p55 subfamily member 4 (MPP4) ELISA Kit employs a two-site sandwich ELISA to quantitate MPP4 in samples. An antibody specific for MPP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	58538

Applications	ELISA
Applications Notes	This Human MAGUK p55 subfamily member 4 (MPP4) ELISA Kit employs a two-site sandwich ELISA to quantitate MPP4 in samples. An antibody specific for MPP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703773	KTE61558	48 T	$339		Add to Cart
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