Human Macrophage Inflammatory Protein-3beta,MIP-3beta ELISA kit

Human Macrophage Inflammatory Protein-3beta,MIP-3beta ELISA kit

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Mfr.No.

CSB-E04670h

Sensitivity

9.75 pg/ml

Detection Range

39 pg/ml-2500 pg/ml

Reactivity

Human

Assay Duration

1-5h

Alternative Names

chemokine (C-C motif) ligand 19, RP11-195F19.11, CKb11, ELC, MGC34433, MIP-3b, MIP3B, SCYA19, CC chemokine ligand 19, CK beta-11, EBI1-ligand chemokine, beta chemokine exodus-3, exodus-3, macrophage inflammatory protein 3-beta, macrophage Inflammatory Protein-3beta,MIP-3beta

Recovery

The recovery of hamster PLBD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	96	89-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of hamster PLBD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-105
1:2	Average %	92
	Range %	86-96
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	91-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.349	2.367	2.358	2.227
400	1.975	1.924	1.950	1.819
200	1.408	1.466	1.437	1.306
100	0.912	0.857	0.885	0.754
50	0.555	0.521	0.538	0.407
25	0.363	0.374	0.369	0.238
12.5	0.248	0.259	0.254	0.123
0	0.133	0.129	0.131	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q99731

Applications	ELISA
Research Area	CCL19

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503252	CSB-E04670h	96T	$468	Add to Cart
BHE10503252	CSB-E04670h	5x96T	$2246.40	Add to Cart
BHE10503252	CSB-E04670h	10x96T	$4446	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.