Human Macrophage Inflammatory Protein-3beta

Mfr.No.

E0085Hu

Sensitivity

2.51ng/L

Detection Range

5-1500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Horse Fibrinogen (also known as Fbg) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Beta-chemokine exodus-3;C-C motif chemokine 19;CCL 19;CCL19;CK beta-11;EBI1 ligand chemokine;ELC;Epstein-Barr virus-induced molecule 1 ligand chemokine;Macrophage inflammatory protein 3 beta;MIP-3-beta;Small-inducible cytokine A19

Molecular Weight

10993

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0085Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	414.3	15.32	3.7
Intra-Assay	2	18	167.1	11.39	6.8
Intra-Assay	3	18	259.1	14.69	5.7

Background

May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

Protocol

These standard curves of E0085Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.137	2.088	2.047
	2.039
400ng/L	1.331	1.347	1.306
	1.362
200ng/L	0.654	0.64	0.6
	0.627
100ng/L	0.52	0.501	0.46
	0.482
50ng/L	0.226	0.212	0.171
	0.198
0ng/L	0.049	0.041	0
	0.032

Gene ID	6363
UniProt ID	Q99731

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101820	E0085Hu	96T	$458		Add to Cart
BHE12101820	E0085Hu	48T	$320		Add to Cart

Human Macrophage Inflammatory Protein-3beta - MIP-3B ELISA Kit

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