Human Macrophage Inflammatory Protein-1alpha,MIP-1alpha ELISA kit

Human Macrophage Inflammatory Protein-1alpha,MIP-1alpha ELISA kit

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Mfr.No.

CSB-E04662h

Sensitivity

9.75 pg/mL

Detection Range

39 pg/mL-2500 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

chemokine (C-C motif) ligand 3, G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3, LD78 alpha beta, small inducible cytokine A3 (homologous to mouse Mip-1a), Macrophage Inflammatory Protein-1alpha,MIP-1alpha, Macrophage Inflammatory Protein-1-alpha,MIP-1-alpha

Recovery

The recovery of human C-MAPT/C-TAU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-95
EDTA plasma (n=4)	100	91-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C-MAPT/C-TAU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	102
	Range %	96-106
1:10	Average %	100
	Range %	90-104
1:20	Average %	95
	Range %	85-99
1:40	Average %	94
	Range %	88-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	3.001	2.889	2.945	2.832
2000	2.114	1.976	2.045	1.932
1000	1.231	1.200	1.216	1.103
500	0.596	0.565	0.581	0.468
250	0.353	0.341	0.347	0.234
125	0.239	0.232	0.236	0.123
62.5	0.171	0.168	0.170	0.057
0	0.111	0.115	0.113

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P10147

Applications	ELISA
Research Area	CCL3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503249	CSB-E04662h	96T	$495	Add to Cart
BHE10503249	CSB-E04662h	5x96T	$2376	Add to Cart
BHE10503249	CSB-E04662h	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.