Human Macrophage Inflammatory Protein-1alpha

Mfr.No.

E0139Hu

Description

This sandwich kit is for the accurate quantitative detection of human Macrophage Inflammatory Protein-1 Alpha (also known as MIP-1A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

C-C motif chemokine 3;CCL 3;CCL3;G0/G1 switch regulatory protein 19-1;LD78-alpha(4-69;Macrophage inflammatory protein 1-alpha;MIP-1-alpha;MIP-1-alpha(4-69;PAT 464.1;SIS-beta;Small-inducible cytokine A3;Tonsillar lymphocyte LD78 alpha protein

Reactivity

Human

Detection Range

1-400ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

10085

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.56ng/L

Background

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

Gene ID	6348
UniProt ID	P10147
Antigen	CCL3

Research Area

Immunology

Protocol

These standard curves of E0139Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/L	2.324	2.343	2.304
	2.362
120ng/L	1.525	1.544	1.505
	1.562
60ng/L	1.139	1.122	1.083
	1.104
30ng/L	0.733	0.752	0.713
	0.771
15ng/L	0.401	0.422	0.383
	0.442
0ng/L	0.037	0.039	0
	0.041

Precision

We measured random samples of E0139Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	84.1	4.75	5.6
Intra-Assay	2	18	52.4	3.08	5.9
Intra-Assay	3	18	30	2.15	7.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101874	E0139Hu	96T	$458		Add to Cart
BHE12101874	E0139Hu	48T	$320		Add to Cart

Human Macrophage Inflammatory Protein-1alpha - MIP-1A ELISA Kit

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