Human Macrophage-Derived Chemokine (MDC/CCL22) ELISA kit

Human Macrophage-Derived Chemokine (MDC/CCL22) ELISA kit

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Mfr.No.

CSB-E04660h

Sensitivity

10.324 pg/mL

Detection Range

15.625 pg/mL-1000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

chemokine (C-C motif) ligand 22, A-152E5.1, ABCD-1, DC/B-CK, MDC, MGC34554, SCYA22, STCP-1, CC chemokine STCP-1, macrophage-derived chemokine, small inducible cytokine A22, small inducible cytokine subfamily A (Cys-Cys), member 22, sti

Recovery

The recovery of rat PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	98-107
EDTA plasma (n=4)	97	92-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	96
	Range %	91-103
1:4	Average %	97
	Range %	91-105
1:8	Average %	94
	Range %	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.655	2.554	2.605	2.516
1000	1.828	1.727	1.778	1.689
500	0.973	0.996	0.985	0.896
250	0.548	0.559	0.554	0.465
125	0.302	0.312	0.307	0.218
62.5	0.244	0.233	0.239	0.150
31.25	0.147	0.142	0.145	0.056
0	0.089	0.088	0.089	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O00626

Applications	ELISA
Research Area	CCL22

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503248	CSB-E04660h	96T	$445	Add to Cart
BHE10503248	CSB-E04660h	5x96T	$2136	Add to Cart
BHE10503248	CSB-E04660h	10x96T	$4227.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.