Human Macrophage Colony-Stimulating Factor,M-CSF ELISA kit

Human Macrophage Colony-Stimulating Factor,M-CSF ELISA kit

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Mfr.No.

CSB-E04658h

Alternative Names

colony stimulating factor 1 (macrophage), MCSF, MGC31930, OTTHUMP00000013363, OTTHUMP00000013364, colony stimulating factor 1, macrophage colony stimulating factor

Reactivity

Human

Detection Range

0.156 ng/ml - 10 ng/ml

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse POMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	98	93-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse POMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	100
	Range %	95-110
1:4	Average %	90
	Range %	88-94
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
3000	0.227	0.219	0.223
750	0.304	0.298	0.301
187.5	0.475	0.456	0.466
46.88	0.752	0.705	0.729
11.72	1.256	1.223	1.240
2.93	1.573	1.614	1.594
0.73	1.811	1.742	1.777
0	2.268	2.297	2.283

Species

Homo sapiens

Sensitivity

0.039 ng/ml

Limit Of Detection

450 nm

UniProt ID	P09603
Antigen	colony stimulating factor 1 (macrophage)

Applications

ELISA

Research Area

CSF1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503246	CSB-E04658h	96T	$395	Add to Cart
BHE10503246	CSB-E04658h	5x96T	$1896	Add to Cart
BHE10503246	CSB-E04658h	10x96T	$3752.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.