Human Mac-2-binding Protein

Mfr.No.

E4379hu

Description

This sandwich kit is for the accurate quantitative detection of human Mac-2-binding Protein (also known as Mac-2BP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

hMTBP;MAC2BP;Mdm2-binding protein;MDM2BP

Reactivity

Human

Detection Range

0.2-60ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

102193

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.092ng/ml

Background

Inhibits cell migration in vitro and suppresses the invasive behavior of tumor cells (By similarity). May play a role in MDM2-dependent p53/TP53 homeostasis in unstressed cells. Inhibits autoubiquitination of MDM2, thereby enhancing MDM2 stability. This promotes MDM2-mediated ubiquitination of p53/TP53 and its subsequent degradation.

Gene ID	27085
UniProt ID	Q96DY7
Antigen	MAC2BP

Research Area

Cancer

Protocol

These standard curves of E4379hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.201	2.142	2.108
	2.082
16ng/ml	1.297	1.32	1.286
	1.342
8ng/ml	0.596	0.669	0.635
	0.741
4ng/ml	0.347	0.384	0.35
	0.42
2ng/ml	0.185	0.173	0.14
	0.161
0ng/ml	0.051	0.033	0
	0.016

Precision

We measured random samples of E4379hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.57	0.35	4.66
Intra-Assay	2	18	19.4	0.57	2.93
Intra-Assay	3	18	9.31	0.4	4.27

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105732	E4379hu	48T	$320		Add to Cart
BHE12105732	E4379hu	96T	$458		Add to Cart

Human Mac-2-binding Protein - M2BP ELISA Kit

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