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Home / ELISA Kit

Human Lysosomal Pro-X carboxypeptidase (PRCP) ELISA Kit

SKU : BHE13703402

Lysosomal Pro-X carboxypeptidase is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein.
The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

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Mfr.No.	KTE61186
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Lysosomal Pro-X carboxypeptidase (PRCP) ELISA Kit employs a two-site sandwich ELISA to quantitate PRCP.
Alternative Names	PRCP; HUMPCP; MGC2202; PCP; angiotensinase C; lysosomal Pro-x carboxypeptidase; lysosomal carboxypeptidase C; proline carboxypeptidase; prolylcarboxypeptidase; prolylcarboxypeptidase isoform 1 preproprotein
Detection Method	Colorimetric
Kit Components	Human Lysosomal Pro-X carboxypeptidase microplate Human Lysosomal Pro-X carboxypeptidase standard Human Lysosomal Pro-X carboxypeptidase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Lysosomal Pro-X carboxypeptidase (PRCP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRCP. No significant cross-reactivity or interference between Human PRCP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Lysosomal Pro-X carboxypeptidase is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Notes	This Human Lysosomal Pro-X carboxypeptidase (PRCP) ELISA Kit employs a two-site sandwich ELISA to quantitate PRCP in samples. An antibody specific for PRCP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRCP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRCP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRCP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5547

Applications	ELISA
Applications Notes	This Human Lysosomal Pro-X carboxypeptidase (PRCP) ELISA Kit employs a two-site sandwich ELISA to quantitate PRCP in samples. An antibody specific for PRCP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRCP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRCP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRCP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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