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Home / ELISA Kit

Human Lysophospholipid acyltransferase 5 (LPCAT3) ELISA Kit

SKU : BHE13704006

The deduced 487-amino acid human and mouse MBOAT5 proteins contain 11 predicted transmembrane domains and share 88% amino acid identity. Human MBOAT5 shares 35% amino acid identity with Drosophila nessy.The deduced protein contains 10 putative transmembrane domains and a C-terminal endoplasmic reticulum (ER) retention motif (KKxx). RT-PCR detected ubiquitous Lpcat3 expression, with highest level in testis. Lpcat3 colocalized with an ER marker protein in transfected Chinese hamster ovary cells.
Knockdown of Lpcat3 in mouse melanoma cells with small interfering RNA significantly reduced cellular acyltransferase activity toward endogenous lysophosphatidylcholine, lysophosphatidylethanolamine, and lysophosphatidylserine.

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Mfr.No.	KTE61791
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Lysophospholipid acyltransferase 5 (LPCAT3) ELISA Kit employs a two-site sandwich ELISA to quantitate LPCAT3.
Alternative Names	LPCAT3; C3F; MBOAT5; OACT5; nessy; O-acyltransferase (membrane bound) domain containing 5; membrane bound O-acyltransferase domain containing 5; putative protein similar to nessy
Detection Method	Colorimetric
Kit Components	Human Lysophospholipid acyltransferase 5 microplate Human Lysophospholipid acyltransferase 5 standard Human Lysophospholipid acyltransferase 5 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Lysophospholipid acyltransferase 5 (LPCAT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human LPCAT3. No significant cross-reactivity or interference between Human LPCAT3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The deduced 487-amino acid human and mouse MBOAT5 proteins contain 11 predicted transmembrane domains and share 88% amino acid identity. Human MBOAT5 shares 35% amino acid identity with Drosophila nessy.The deduced protein contains 10 putative transmembrane domains and a C-terminal endoplasmic reticulum (ER) retention motif (KKxx). RT-PCR detected ubiquitous Lpcat3 expression, with highest level in testis. Lpcat3 colocalized with an ER marker protein in transfected Chinese hamster ovary cells. Knockdown of Lpcat3 in mouse melanoma cells with small interfering RNA significantly reduced cellular acyltransferase activity toward endogenous lysophosphatidylcholine, lysophosphatidylethanolamine, and lysophosphatidylserine.
Notes	This Human Lysophospholipid acyltransferase 5 (LPCAT3) ELISA Kit employs a two-site sandwich ELISA to quantitate LPCAT3 in samples. An antibody specific for LPCAT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPCAT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPCAT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPCAT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10162

Applications	ELISA
Applications Notes	This Human Lysophospholipid acyltransferase 5 (LPCAT3) ELISA Kit employs a two-site sandwich ELISA to quantitate LPCAT3 in samples. An antibody specific for LPCAT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPCAT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPCAT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPCAT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704006	KTE61791	48 T	$339		Add to Cart
BHE13704006	KTE61791	96 T	$589		Add to Cart
BHE13704006	KTE61791	96 Tx5	$2629		Add to Cart
BHE13704006	KTE61791	96 Tx50	$24569		Add to Cart

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