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Home / ELISA Kit

Human Lysophosphatidylcholine acyltransferase 1 (LPCAT1) ELISA Kit

SKU : BHE13704008

Lysophosphatidylcholine (LPC) acyltransferase (LPCAT) catalyzes the conversion of LPC to phosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis.
deduced 534-amino acid mouse and human proteins contain 3 transmembrane domains, several motifs conserved in members of the LPCAT family, a putative EF hand domain, and a C-terminal endoplasmic reticulum (ER) retention signal. They share about 88% amino acid identity. Real-time RT-PCR of mouse tissues showed that Lpcat1 was expressed at highest levels in lung, followed by spleen and brain. Other tissues had low Lpcat1 levels. Confocal microscopy detected epitope-tagged Lpcat1 mainly in the ER and Golgi of transfected cells.

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Mfr.No.	KTE61793
Description	This Human Lysophosphatidylcholine acyltransferase 1 (LPCAT1) ELISA Kit employs a two-site sandwich ELISA to quantitate LPCAT1.
Alternative Names	LPCAT1; AYTL2; FLJ12443; FLJ41609; PFAAP3; lpcat; LPC acyltransferase; acyl-CoA:lysophosphatidylcholine acyltransferase 1; acyltransferase like 2; phosphonoformate immuno-associated protein 3; regulated by phos
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Lysophosphatidylcholine acyltransferase 1 microplate Human Lysophosphatidylcholine acyltransferase 1 standard Human Lysophosphatidylcholine acyltransferase 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Lysophosphatidylcholine acyltransferase 1 (LPCAT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human LPCAT1. No significant cross-reactivity or interference between Human LPCAT1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Lysophosphatidylcholine (LPC) acyltransferase (LPCAT) catalyzes the conversion of LPC to phosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis. deduced 534-amino acid mouse and human proteins contain 3 transmembrane domains, several motifs conserved in members of the LPCAT family, a putative EF hand domain, and a C-terminal endoplasmic reticulum (ER) retention signal. They share about 88% amino acid identity. Real-time RT-PCR of mouse tissues showed that Lpcat1 was expressed at highest levels in lung, followed by spleen and brain. Other tissues had low Lpcat1 levels. Confocal microscopy detected epitope-tagged Lpcat1 mainly in the ER and Golgi of transfected cells.
Notes	This Human Lysophosphatidylcholine acyltransferase 1 (LPCAT1) ELISA Kit employs a two-site sandwich ELISA to quantitate LPCAT1 in samples. An antibody specific for LPCAT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPCAT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPCAT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPCAT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	79888
Antigen	LPCAT1
Tag	LPCAT1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Lysophosphatidylcholine acyltransferase 1 (LPCAT1) ELISA Kit employs a two-site sandwich ELISA to quantitate LPCAT1 in samples. An antibody specific for LPCAT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPCAT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPCAT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPCAT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704008	KTE61793	48 T	$339		Add to Cart
BHE13704008	KTE61793	96 T	$589		Add to Cart
BHE13704008	KTE61793	96 Tx5	$2629		Add to Cart
BHE13704008	KTE61793	96 Tx50	$24569		Add to Cart

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