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Home / ELISA Kit

Human Lysophosphatidic acid (LPA) ELISA Kit

SKU : BHE13704741

Lipoprotein(a) consists of an LDL-like particle and the specific apolipoprotein(a) , which is covalently bound to the apoB of the LDL like particle. Lp(a) plasma concentrations are highly heritable and mainly controlled by the apolipoprotein(a) gene located on chromosome 6q26-27. Apo(a) proteins vary in size due to a size polymorphism , which is caused by a variable number of so called kringle IV repeats in the LPA gene. This size variation at the gene level is expressed on the protein level as well, resulting in apo(a) proteins with 10 to > 50 kringle IV repeats . These variable apo(a) sizes are known as "apo(a) isoforms". There is a general inverse correlation between the size of the apo(a)isoform and the Lp(a) plasma concentration which is caused by a variable rate of degradation before the apo(a) protein has matured for Lp(a) assembly.

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Mfr.No.	KTE62534
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Lysophosphatidic acid (LPA) ELISA Kit employs a two-site sandwich ELISA to quantitate LPA.
Alternative Names	LPA
Detection Method	Colorimetric
Kit Components	Human Lysophosphatidic acid microplate Human Lysophosphatidic acid standard Human Lysophosphatidic acid detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Lysophosphatidic acid (LPA) ELISA Kit has high sensitivity and excellent specificity for detection of Human LPA. No significant cross-reactivity or interference between Human LPA and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Lipoprotein(a) consists of an LDL-like particle and the specific apolipoprotein(a) , which is covalently bound to the apoB of the LDL like particle. Lp(a) plasma concentrations are highly heritable and mainly controlled by the apolipoprotein(a) gene located on chromosome 6q26-27. Apo(a) proteins vary in size due to a size polymorphism , which is caused by a variable number of so called kringle IV repeats in the LPA gene. This size variation at the gene level is expressed on the protein level as well, resulting in apo(a) proteins with 10 to > 50 kringle IV repeats . These variable apo(a) sizes are known as "apo(a) isoforms". There is a general inverse correlation between the size of the apo(a)isoform and the Lp(a) plasma concentration which is caused by a variable rate of degradation before the apo(a) protein has matured for Lp(a) assembly.
Notes	This Human Lysophosphatidic acid (LPA) ELISA Kit employs a two-site sandwich ELISA to quantitate LPA in samples. An antibody specific for LPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Lysophosphatidic acid (LPA) ELISA Kit employs a two-site sandwich ELISA to quantitate LPA in samples. An antibody specific for LPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704741	KTE62534	48 T	$339		Add to Cart
BHE13704741	KTE62534	96 T	$589		Add to Cart
BHE13704741	KTE62534	96 Tx5	$2629		Add to Cart
BHE13704741	KTE62534	96 Tx50	$24569		Add to Cart

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